![]() C and D Western blotting to detect GPD1 expression in 4 paired normal and bladder tumor tissue. B Volcano plot of differentially expressed proteins between bladder cancer tissues and matched adjacent normal tissues. A The heatmap shows significantly upregulated or downregulated proteins between bladder cancer tissues and matched adjacent normal tissues. ![]() Proteomics reveals low expression of GPD1 in human bladder cancer tissues. Pharmacological activation of GPD1 is a potential therapeutic approach for bladder cancer.Īllosteric activator Bladder cancer GPD1 Metabolic enzyme Tumor suppressor. This study suggests that GPD1 may act as a novel tumor suppressor in bladder cancer. Finally, we performed a virtual screening to obtain the GPD1 allosteric activator wedelolactone and demonstrated its ability to inhibit bladder tumor growth in vitro and in vivo. Based on transcriptomics and metabolomics, GPD1 promotes Ca 2+ influx and apoptosis of tumor cells via the lysoPC-PAFR-TRPV2 axis. Further investigation showed that GPD1 overexpression significantly promoted apoptosis in bladder cancer cells. Here, we used proteomics to find that GPD1 expression was at low levels in bladder cancer tissues. Virtual screening was used to identify allosteric activator of GPD1. Transcriptomics and metabolomics were performed to reveal the underlying mechanisms of GPD1. The function of GPD1 on bladder cancer cells were confirmed through in vivo and in vitro assays. Proteomics were performed to compare protein expression differences between human bladder cancer tissues and adjacent normal tissues. ![]() There is a need to further explore more effective targeted therapeutic strategies. ![]() ![]() However, the outcomes are not satisfactory for patients with advanced bladder cancer. Surgical resection and chemotherapy are the two mainstream treatments for bladder cancer. Bladder cancer is the most common malignant tumor of the urinary system. ![]()
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