When concentration = 0 there is no peak to integrate, so there may be merely a missing value (which has no error associated with it). Generally you cannot handle your X=0 point in the same way as all the others, because the others have a measured value for Y. (3) There was a big article in LC-GC a few months ago on how to handle zero points. (2) Any chromatography system worth its salt can do weighted least squares, so you should probably do it with the software that came with your hplc (assuming you are not using an old system without software control). I have not tried this site and cannot endorse whether it's safe, but good luck! Which give hints and downloads how it might be done. However, a quick google search reveals sites such as: (1) Excel, as it stands, does not do weighted least squares regressions. Please provide information to the concerned questions. How do these statistical softwares consider weighting in such a case? Will these softwares ignore the point (x,y) when x=0? What is the right thing to do in such a scenario? In such a case, how is the weighting considered when x=0 ? I have this question because 1/x, 1/x/x cannot be defined when x=0. Also, in my research i will be having some baseline value of "Y" when X=0 (for example, for x=0, y = 10 units). ? Are there any free Statistical softwares available for drawing these best fit lines?Ģ. How can i obtain a best fit line if i use a weighting scheme such as 1/x, 1/x/x, etc. For different values of X and Y, I can obtain a best fit line in Microsoft Excel without using any weighting. Suppose i have an independent variable X (concentration) and a dependent variable Y (peak area). Please share your opinions regarding my following questions.ġ. I have a couple of questions regarding weighted linear regression that i will be using for the calibration curves.
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